Since the COVID-19 pandemic, supply chain issues in the biomanufacturing sphere have meant that the traditional approach to the production of biologicals, for example monoclonal antibodies has become considerably more expensive. Optimisation of both the upstream and downstream operations requires a new approach. Furthermore, with the advent of larger bioentities such as Adeno Associated Virus (AAV) and Lentivirus (LV) these traditional methods result in very low recovery of the target entity and, as a direct result, much higher production costs.
SMART Chromatography™ removes the necessity to clarify the target containing feed stream before application to the primary capture column. The whole, undiluted, unmodified feed stream is passed through the column, thus combining the unit operations of clarification and primary purification into a single, linearly-scalable step.
The result is higher recovery of the biomolecule that is produced in the bioreactor, improved retention of biological activity, lower overhead costs (personnel, facility, etc.) and faster processes.
In this presentation/poster we will present data on the purification from different cell systems including CHO, P. pastoris and Dyadic’s C1 filamentous fungi.