F1Fo ATP synthase has a vital role in bioenergetic pathways in translating a proton motive force into a rotational force, driving ATP synthesis. As such, new classes of antibiotics targeting ATP synthase are of interest in developing treatments against multi drug resistant bacteria. Pseudomonas aeruginosa is an opportunistic pathogen responsible for severe infections in immunocompromised individuals. We aim to purify and solve the high-resolution structures of ATP synthase from P. aeruginosa, both in apo and drug bound forms. These structures will aid in inhibitor design for future treatments of this pathogen.